Monitoring Aquatic Biodiversity: DNA Barcoding for Invasive Species Detection in the Hudson River

Authors: Elizabeth Hemway, Shalini Varma, Rana Ayvaz, Riley Schwartz, Mathew Mead, Jax A Skylstad, Teagan Shea, Crystal Edwards, Kaitlyn M O'Neil

SUNY Campus: Hudson Valley Community College

Presentation Type: Poster

Location: Old Union Hall

Presentation #: 50

Timeslot: Session B 10:15-11:15 AM

Abstract: The Hudson River, a vital waterway in New York, is home to diverse ecosystems that are increasingly threatened by invasive species. Early detection and management of these invasive species are essential for the preservation of native biodiversity. DNA barcoding offers a powerful tool for species identification, providing an efficient and accurate method for detecting both aquatic and terrestrial organisms in environmental samples. By harnessing genetic material released by organisms into their environment, eDNA provides an efficient means of monitoring aquatic ecosystems for the presence of targeted invasive species. This study focuses on employing DNA barcoding as an innovative and precise method for the detection of two invasive species, round goby (Neogobius melanostomus) and water chestnuts (Trapa natans). In this pilot study, we collected water and sediment samples from various locations along the Hudson River and extracted DNA to identify species through a standardized genetic marker (e.g., COI gene for animals, rbcL for plants). We were able to isolate DNA from various samples collected and PCR was performed. Subsequent DNA sequencing and bioinformatics analysis will enable the identification of invasive species through comparison with established genetic databases. This study highlights the potential of DNA barcoding for enhancing the detection and management of invasive species in aquatic environments, ultimately aiding conservation efforts in the Hudson River ecosystem. The findings will contribute to a comprehensive understanding of the invasive species dynamics in the Hudson River, laying the foundation for sustainable protection and preservation of the Hudson River ecosystem.