How do Antiseptics Play a Role in the Development of Resistant Bacteria?

Authors: Justice Crane, Michel Pelletier

SUNY Campus: SUNY Brockport

Presentation Type: Poster

Location: Old Union Hall

Presentation #: 43

Timeslot: Session D 3:00-4:00 PM

Abstract: The human skin microbiota consists of a diverse range of microorganisms that play crucial roles in pathogen defense and immune system regulation. The widespread use of antiseptics, such as iodine and triclosan, is intended to reduce pathogenic microorganisms and prevent infections. However, overuse of these antiseptics may lead to the development of resistance in skin bacteria. This project investigates whether antiseptics contribute to the development of resistance in skin bacteria, focusing on iodine and triclosan. Bacteria were isolated from skin and teeth samples using selective growth media, and their susceptibility to antiseptics was assessed by exposing them to increasing concentrations of the disinfectants. The minimum bactericidal concentration (MBC) was determined, and bacteria were subsequently grown in the MBC concentrations to select for resistant strains. Staphylococcus capitis showed resistance to triclosan. A biofilm assay was performed to examine if resistant bacteria exhibited an enhanced ability to form biofilms, which can increase virulence. The results showed a significant increase in optical density (OD) of biofilms formed by resistant strains, with a 148% increase in OD compared to wild-type S. capitis. This indicates that bacteria, such as S. capitis, can mutate to become resistant to triclosan. Given the widespread use of antiseptics in healthcare and daily life, these findings highlight the potential risks of overusing antiseptics and the need for alternative strategies to combat bacterial resistance.