Investigating Microbial Diversity in Long Island Watersheds: Marine Nature Preserve

Authors: Iqra Saeed, Zulema Cabail

SUNY Campus: SUNY Old Westbury

Presentation Type: Poster

Location: Old Union Hall

Presentation #: 48

Timeslot: Session C 1:45-2:45 PM

Abstract: This study is part of the ongoing Watershed Analysis Project, conducted as a Course-Based Undergraduate Research Experience (CURE) in the Basic Biological Science I Lab. The project aims to assess microbial diversity—specifically plankton and bacterial communities—as an indicator of water quality in Long Island watersheds and to explore how environmental factors influence their distribution. During the Fall 2024 semester, we conducted a four-part investigation: 1. Field Sampling: Water samples were collected from the Marine Nature Preserve (Oceanside, NY) and Wantagh Park (Wantagh, NY) in October 2024. We documented the collection sites and measured key chemical and physical parameters, including temperature, ammonia levels, and salinity. 2. Microscopic Identification: One liter of water was filtered through a 2-micron filter to concentrate plankton, which were then identified using microscopy. 3. DNA Extraction and Sequencing: A second liter of water was used for DNA extraction from the microbial community. Samples were sent to Integrated Microbiome Resources for 16S and 18S rRNA gene sequencing, which allowed us to identify bacterial and planktonic diversity. 4. Bioinformatics Analysis: We analyzed 16S rRNA sequences to compare microbiome diversity across collection sites. This research contributes to a broader understanding of microbial ecology in Long Island watersheds. I will present findings specifically from the Marine Nature Preserve, highlighting variations in microbial diversity and potential environmental influences. In future research, we will analyze 18S rRNA sequences.