Effects of (1, 4 DPCA) on human osteoblastic cells

Authors: T Maphosa, Rosemary Dziak

SUNY Campus: SUNY Buffalo

Presentation Type: Poster

Location: Old Union Hall

Presentation #: 31

Timeslot: Session C 1:45-2:45 PM

Abstract: When there is a disease such as periodontal disease or osteoporosis that leads to bone loss, osteoblastic cells need to be activated to support bone healing and restoration. There are many factors involved in the regulation of osteoblasts and drugs such as 1,4-dihydrophenonthrolin-4-one-3-carboxylic acid (1, 4 DPCA have been shown to have potential for regeneration. However, more research is needed for effective administration of the drug to sites where tissue restoration is needed. The focus here is on the use of hydrogels, as a carrier for the drug and strontium ions and a scaffold for improved osteoblastic cellular metabolism and function. Human osteoblastic cells obtained from a commercial supplier were cultured. An MTT assay was used to measure cellular activity. Experimental groups were made with different combinations of 1,4 DPCA, strontium citrate, and appropriate control solvents. A sample size of 4 was used for each group. Data, spectrophotometric absorbance readings, were statistically analyzed by ANOVA. The group with all additions (cells, drug, and strontium) should have the largest increase in cell activity. 1,4 DPCA, in a hydrogel scaffold with strontium added as a promoter for cell activity could have potential as a tissue regenerative device.