Natural Polymorphisms in Genes Involved in Neural Development Associated with Longevity in Drosophila melanogaster

Authors: Neel Singh, Ruth Duncan, James Tarpey, Robert Dalipovic, Anthony Fiumera

Field of Study: Biological Sciences; Environmental Studies

Program Affiliation: Undergraduate Research Award

Faculty Mentors: Anthony Fiumera

Easel: 87

Timeslot: Afternoon

Abstract: Understanding the mechanisms underlying longevity is a central goal of biomedical research. Drosophila melanogaster is an ideal model for uncovering aging‑related mechanisms, yet the genetic basis of natural variation in survival remains poorly defined. Our goal was to identify lifespan‑associated loci by leveraging natural genetic variation within a panel of 33 inbred DGRP lines. This study measured survival in 430 females and performed a GWAS. This research identified polymorphisms in nine genes associated with survival, including Pumilio, CG42594, and N‑cadherin 2 (CadN2), which are involved in neuronal development. Pumilio is an RNA‑binding protein, and putative Pumilio‑binding sites in CG42594 and CadN2 were identified, suggesting post‑transcriptional regulation. External datasets highlight CadN2 as a high‑priority Pumilio target, with expression varying across Pumilio genotypes. Ultimately, these results suggest that variation in Pumilio-mediated translational control may coordinate neural development, thereby affecting survival. Investigating this regulatory axis may reveal conserved mechanisms in humans.