An Ancient Medicament is Effective in Inhibiting the Growth of S. aureus and P. aeruginosa

Authors: Noah Walsh, Alyssa Millet, Hillary Becker, Cláudia N.H. Marques

Field of Study: Biological Sciences

Faculty Mentors: Cláudia N.H. Marques, Hillary Becker

Easel: 73

Timeslot: Midday

Abstract: Pedanius Dioscorides, the “father” of pharmacognosy, describes using ground narcissus bulbs with honey to treat burns. Honey is an invaluable treatment for wounds in many cultures, and has broad antimicrobial spectrum activity due to peroxide production, low pH and water activity, antimicrobial peptides, and phenolic compounds. The Narcissus genus produces several unique alkaloids with antibacterial activity against S. aureus, one of the most common pathogens found in infected burns. This work evaluated the properties of this ancient medicament against S. aureus and P. aeruginosa (another common culprit in infected burns). These microorganisms were cultured in decreasing concentrations of honey, narcissus oil, or both to establish a minimum inhibitory concentration (MIC). Combining honey and narcissus oil decreased the MIC in both species, indicating the medicament has a synergistic effect in inhibiting these microorganisms. With the increasing need of antibiotic alternatives, this medicament may be an effective alternative in the modern day.